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How to Calculate Kcat.
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How to Calculate Kcat.

Rosemary Njeri

Kcat is the turnover number that describes the number of times each enzyme site converts substrate to product per unit time.

A Kcat of 5/second means that that enzyme makes five molecules of product per molecule of enzyme per second.

Formula to calculate Kcat.

How to Calculate Kcat.

ET is the total enzyme concentration.

Example:

If the Vmax of an enzyme is 30mols/sec, calculate the Kcat if the ET is 6mols.

How to Calculate Kcat.

Therefore, your Kcat is 5/sec.

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