Kcat is the turnover number that describes the number of times each enzyme site converts substrate to product per unit time.

A Kcat of 5/second means that that enzyme makes five molecules of product per molecule of enzyme per second.

**Formula to calculate Kcat.**

ET is the total enzyme concentration.

**Example:**

If the Vmax of an enzyme is 30mols/sec, calculate the Kcat if the ET is 6mols.

Therefore, your Kcat is 5/sec.