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How to Calculate Concentration from Absorbance.
Chemistry

How to Calculate Concentration from Absorbance.

Rosemary Njeri

Absorption means that a substance captures and transforms energy while concentration refers to the amount of a substance in a defined space.

If the concentration of solution is increased, then there are more molecules for the light to hit when it passes through.

Formula to calculate concentration from absorbance.

Calculate Concentration from Absorbance.

Example:

Suppose the molar absorptivity of Na Cl is 193L mol-1 cm-1 and the length of its light path is 5 cm, calculate the concentration if the absorbance is 200.

Calculate Concentration from Absorbance.

Therefore, the concentration is 0.21M.

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